Trichoderma virideのCellulolytic Complexについて : (第6報)Complex各成分の分離, 精製および性質

概要

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A commercial enzyme preparation, Cellulaase onozuka P1500 derived from Trichoderma viride, was purified. The non-adsorbed fraction of the preparation gained by using the gauze column method was fractionated with DEAE-Sephadex A-50,Amberlite CG-50,and Sephadex G-100,giving rise to two components. One of these was electrophoretically almost homogeneous. These two components, consisting mainly of CMCase activity, revealed no activities capable of degrading filter paper and absorbent cotton, but showed trace activity against Avicel. After purificatioin of the adsorbed fraction by a similar method, three electrophoretically homogeneous components were recognized. Of these, one component strongly decomposed filter paper, Avicel, and absorbent cotton, as well as CMC; thus, this component was considered to be an essential component of the natural cellulose decomposing activity of T. viride. Cellotriose, cellotetraose, and swollen cellulose were decomposed by all five components. Molecular weight of these cellulolytic components ranged from 30,000 to 60,000; random hydrolysis of CMC was demonstrated paper chromatographically by using each component.
公益社団法人日本生物工学会の論文
1972-04-25