畳における抗菌剤の評価方法

概要

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Aspergillus sp. was isolated from the Tatami. This fungal spore suspension 100μ l was inoculated on to the potato dextrose agar. As for the antimicrobial agent used for the test, a precision amount was sprayed on the Tatami cut off in all directions of 4.5 cm. After Tatami was dried, it was put in center of the culture medium, and then cultured in 28 ℃. Fungal growth was observed everyday, and the days (Y) until the inhibition zone formed around the Tatami disappears, the days (Z) until mycelium covered whole surface of the Tatami were seeked. When the amount of the antimicrobial agent was decided with X(ml), the titer of antimicrobial agent was designed as (Y+Z)/X. 畳より、Aspergillus.を分離した.本菌の胞子懸濁液10(6)個/ml,100μ1をポテトデキストロース寒天培地に塗布した.4.5cm四方に切り取った畳表に供試する抗菌剤を正確量噴霧し,乾燥後,培地の中心部に置き28℃で培養した.1日毎菌の生育を観察し,畳表周辺に形成される増殖阻止円が消失するまでの日数(Y),菌糸が畳表の全面を覆うまでの日数(Z)を求めた.抗菌剤の噴霧量をX(m1)とすると抗菌剤の力価を(Y+Z)/Xで表わすことを考案した.
宮崎大学の論文