細胞分離酵素とCellulaseによる甘藷澱粉の製造 : (第2報)酵素剤を用いる乾式澱粉製造法

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An attempt was made to isolate starch from sweet potatoes using a cell separating enzyme (protopectinase) preparation derived from a species of Rhizopus along with a cellulase preparation derived from Trichoderma viride or Aspergillus niger. Two processes were developed.(1) Raw sweet potato was crushed without the addition of water and separated into starch slurry and starch waste by a continuous juicer, followed by centrifuging of the slurry to isolate the starch. By a ratio of enzyme solution to substrate of 1 : 1 to 1.5 : 1 by weight, a 0.5 percent solution of mixed (1 : 1) enzyme preparation was added to the starch waste. After one hour incubation while stirring at 500 rpm, 40℃, pH 4.5,a similar starch yield was gained to that obtained in the usual process.(2) Sliced and air-dried sweet potatoes were disintegrated and fractionated by sifting into a 200 mesh powder and residue. By a ratio of 2 : 1,a 0.25 percent solution of mixed enzyme preparation was added to the powder and by a ratio of 3 : 1,a 0.33 percent enzyme solution to the residue. After a four hour incubation while stirring at 300 rpm, 40°C, pH 4.5,the rate of starch recovery was 76 percent in the case of powder, while the rate of recovery from residue was only 32 percent though 4 times the amount of starch was recovered by the treatment using enzyme as compared with the control.(3) In both processes, a small quantity of enzyme preparation, about 0.3 percent of the weight of the raw sweet potato, was sufficient to show its activity. The quantity of water used for starch isolation, purification and washing was half as much as for the usual process.
社団法人日本生物工学会の論文
1966-10-25

細胞分離酵素とCellulaseによる甘藷澱粉の製造 : (第2報)酵素剤を用いる乾式澱粉製造法

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