醸造に対するセルラーゼの応用 : (第4報)セルラーゼと細胞分離酵素の併用による大豆蛋白質の分離

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The cotyledon of soybean consists of numerous unicells containing protein and oil. Those are surrounded with a thick cell wall and these unicells adhere to each other mainly with protopectin. A cell separating enzyme derived from a mold Rhizopus sp. including principally protopectinase is capable of degrading seedcoat and of producing unicells, while cell walls composed substantially of cellulose are decomposed with a cellulase preparation derived from the mold Trichoderma viride.Attempts were made to isolate soybean protein by using both enzyme preparations from air-dried soybeans and defatted soybean cakes. After stirring soaked soybeans and crushed soybean cakes at 40℃, 250 to 300rpm, pH 5.0 to 6.5,for 3 to 5hr with enzyme solution containing a cell separating enzyme and cellulase by a ratio of 1 : 1 by weight, the hydrolyzate was squeezed by nylon filter cloth (plain fabrics, 200 mesh/cm^2) after which the filtrate was separated into protein and supernatant by centrifuging at 3400rpm, pH 4.5 for 10min.It was shown to be adequate to use a mixed enzyme solution at concentrations of 0.2 to 1.0 percent (usually 1.0 to 3.0 percent of substrate). Although good results were obtained by enzymic treatment of raw soybeans and defatted soybean cakes with a nitrogen solubility index of 24,the protein could be isolated from defatted soybean cakes with a nitrogen solubility index of 80 readily by treatment with hot water only.
社団法人日本生物工学会の論文
1966-11-25

醸造に対するセルラーゼの応用 : (第4報)セルラーゼと細胞分離酵素の併用による大豆蛋白質の分離

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