Xanthomonas campestris pv. citri の産生するバクテリオシン様物質の精製と若干の性質

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An antibacterial substance released from cells of Xanthomonas campestris pv. citri Ku 7508 by chloroform treatment was purified and its several properties were examined. The substance was not precipitated by centrifuging even at 60,000×g for 60 min, partially precipitated at 75 % acetone (final concentration) and completely precipitated at 60 % saturation with ammonium sulfate. It was not dialysed through Visking tube and inactivated by heating at 60 C for 30 min. The purification of this substance was conducted by DEAE ion-exchange cellulose column followed by Ultrogel column. Antibacterial activity of eluates was corresponded with the peak of UV absorbance at 280 nm. A typical UV spectrum of protein, i. e., maximum at 280 nm and minimum at 250 nm was observed. Two or three bands were detected by Coomassie-blue staining at disc gel electrophoresis of this active fraction. One of the band having antibacterial activity was sliced and repeatedly subjected to disc gel electrophoresis and single band was isolated. Molecular weight of this substance estimated by SDS-polyacrylamide gel electrophoresis was about 300,000 daltons. Antibacterial substance caused retardation of bacterial growth due to aggregation or disruption of cells during the first several hr. The effet, however, disappeared within 24 hr of incubation. Electron microscopic observation revealed that the antibacterial substance caused leaking of bacterial cytoplasm due to disruption of cell wall. These results suggest that the antibacterial substance was a kind of bacteriocin.
日本植物病理学会の論文
1981-09-25

Xanthomonas campestris pv. citri の産生するバクテリオシン様物質の精製と若干の性質

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