Selective Cadmium Accumulation Using Recombinant Escherichia coli (Environmental Biotechnology)
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概要
- 論文の詳細を見る
Recombinant Escherichia coli JM109 (pZH3-5/pMT), harboring a manganese transport gene (mntA) and a metal-sequestering protein (metallothionein [MT]) gene, was cultivated to accumulate cadmium (Cd) in an aqueous phase. Isopropyl β-D-thiogalactoside (IPTG)-induced cells showed rapid Cd^<2+> ion accumulation (90% of maximum accumulation in 15 min) and had an accumulation six times higher than that of the control. Under optimum conditions, i.e., pH 7, 37℃ and 0.5 (OD_<600>), 1.5 mM IPTG induction resulted in the accumulation of 21.5 μmol Cd/g dry cell. Storage at 37℃ for 24 h had no effect on the accumulation. Significantly, Cd was selectively accumulated in a solution containing an equal concentration of three other metals, resulting in more than 90% of the total accumulated metals being Cd. The accumulation of Cd was reduced by the presence of Mn^<2+> ion whereas no significant effect was observed with Cu^<2+>, Zn^<2+> and Pb^<2+> ions. A chelator, EDTA, had no effect on the accumulation up to 100 mM. The bioaccumulation rate followed Michaelis-Menten kinetics (V_m=2.7 μmol Cd^<2+>/min・g dry cell, K_m=0.67 μM). The equilibrium isotherm showed a Langmuir isotherm. In the membrane reactor experiment, 1 mg/l Cd in an inlet solution decreased to 0.2 mg/l in the effluent, removing 80% of Cd, continuously. These results indicated the potentials of a genetically modified microorganism for the highly selective accumulation of Cd at a low concentration and the future application to the removal and recovery of Cd.
- 社団法人日本生物工学会の論文
- 2005-02-25
著者
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Kim Se
Department of Radiology, Seoul National University Hospital
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KIM Eun
Department of Phpysics, Hanyang University
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Kim Se
Department Of Anatomy College Of Veterinary Medicine Chonnam National University
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Kim Eun
Department Of Biological Engineering Inha University
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Kim Se
Department Of Biological Engineering Inha University
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LEE BAEK
Department of Biological Engineering, Inha University
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WILSON DAVID
Department of Molecular Biology and Genetics, Biotech, Building, Cornell University
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Wilson David
Department Of Molecular Biology And Genetics Biotech Building Cornell University
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Wilson David
Department Of Anatomy School Of Medicine The Queen's University Of Belfast
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Lee Baek
Department Of Biological Engineering Inha University
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Kim Eun
Department Of Anesthesia And Pain Medicine School Of Medicine Pusan National University
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Kim Se-Kwon
Department of Biological Engineering, Inha University
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Lee Baek-Suk
Department of Biological Engineering, Inha University
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KIM Eun
Department of Agricultural Biotechnology, Seoul National University
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