マイクロメカニカルデバイスを用いた1対1細胞融合技術の開発

概要

論文の詳細を見る
A cell fusion technique using micromechanical silicon devices has been developed. It permits a number of one-to-one cell fusion operations between two different biological cell groups to be carried out simultaneously, instead of the conventional collective fusion operation. A matrix array of 1584 microchambers, each containing a single pair of cells, have been fabricated on a 3-inch silicon wafer. Fusion of the paired cells is induced by electric pulses applied between electrodes embedded in the microchambers. In order to feed a pair of cells from two different cell groups to every microchamber, a cell carrier made of a silicon wafer has been developed. A single cell particle is absorbed to and released from the port of the carrier plate. The carrier plate is transferred over the microchamber plate and simultaneously releases cells into the microchambers. Repeating this operation independently for the cell groups A and B, a single pair of AB is formed in every microchamber. It is observed that pairs of lettuce cells are successfully fused in the microchambers.
公益社団法人精密工学会の論文
1990-01-05