A Rapid and Reliable Solid-Phase Extraction Method for High-Performance Liquid Chromatographic Analysis of Opium Alkaloids from Papaver Plants
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概要
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A rapid and reliable solid-phase extraction method for HPLC analysis of opium alkaloids from Papaver plants was established. Fifty mg of dried and powdered plant sample was extracted with 5ml of 5% acetic acid for 30 min under sonication. After centrifugation, 3ml of the supernatant was loaded on a reversed-phase cation-exchange solid-phase extraction cartridge. After seriate washings with 0.1M hydrochloric acid and methanol, alkaloids were eluted with a mixture of 28% ammonia and methanol (1:19). The eluate was concentrated under nitrogen stream at 40℃ and the residue was dissolved in 50% aqueous methanol for high performance liquid chromatographic analysis. With this solid-phase extraction method, the recovery of morphine, codeine, oripavine, thebaine, papaverine, noscapine and sanguinarine was from 99.94 to 112.18% when the standard alkaloids were added to the plant samples. Opium alkaloids of a variety of genus Papaver plants cultivated in a field and phytotron were analyzed by this method.
- 公益社団法人日本薬学会の論文
- 2005-11-01
著者
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KIUCHI Fumiyuki
Research Center for Medicinal Plant Resources National Institute of Biomedical Innovation
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YOSHIMATSU Kayo
Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation
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Kiuchi F
Graduate School Of Pharmaceutical Sciences Kyoto University
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Kiuchi Fumiyuki
国立医薬品衛生研究所
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Kikuchi F
Faculty Of Pharmaceutical Sciences Kanazawa University:(present Address)graduate School Of Pharmaceu
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Kikuchi F
Faculty Of Pharmaceutical Sciences Kanazawa University
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Kiuchi Fumiyuki
National Inst. Biomedical Innovation Ibaraki Jpn
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SHIMOMURA Koichiro
Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation
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MAKINO Yukiko
Narcotics Control Department, Kanto-Shin'etsu Regional Bureau of Health and Welfare
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Kiuchi Fumiyuki
Graduate School Of Pharmaceutical Sciences Kyoto University
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Shimomura Koichiro
Tsukuba Medicinal Plant Research Station National Institute Of Health Sciences:faculty Of Life Scien
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Makino Yukiko
Narcotics Control Department Kanto-shin'etsu Bureau Of Health And Welfare Ministry Of Health La
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Yoshimatsu K
National Inst. Biomedical Innovation Ibaraki Jpn
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Yoshimatsu K
National Inst. Health Sci. Ibaraki Jpn
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Yoshimatsu Kayo
Research Center For Medicinal Plant Resources National Institute Of Biomedical Innovation
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Kiuchi Fumiyuki
Faculty Of Pharmaceutical Sciences Kanazawa University:(present Address)graduate School Of Pharmaceu
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Makino Yukiko
Narcotices Control Department Kanto-shin'etsu Regional Bureau Of Health And Welfare Ministry Of Health Labour And Welfare
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