Biomimetic Trehalose Biosensor Using Gustatory Receptor (Gr5a) Expressed in Drosophila Cells and Ion-Sensitive Field-Effect Transistor

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概要

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• The development of potential applications of biosensors using the sensory systems of vertebrates and invertebrates has progressed rapidly, especially in clinical diagnosis. The biosensor developed here involves the use of Drosophila cells expressing the gustatory receptor Gr5a and an ion-sensitive field-effect transistor (ISFET) sensor device. Gustatory receptor Gr5a is expressed abundantly in gustatory neurons and acts as a primary marker for tastants, especially sugar, in Drosophila. As a result, it could potentially serve as a good candidate for potential biomarkers of diseases in which the current knowledge of the cause and treatment is limited. The developed ISFET was based on the outstanding electrical characteristics of the metal--oxide--semiconductor field-effect transistor (MOSFET) with a subthreshold swing of 85 mV/dec, low leakage current of {<}10^{-12} and high on/off current ratio of 7.3\times 10^{6}. The SiO<inf>2</inf>sensing membrane with a pH sensitivity of 34.9 mV/pH and drift rate 1.17 mV/h was sufficient for biosensing applications. In addition, the sensor device also showed significant compatibility with the Drosophila cells expressing Gr5a and their response to sugar, particularly trehalose. Moreover, the interactions between the transfected Drosophila cells and trehalose were consistent and reliable. This suggests that the developed ISFET sensor device could have potential use in the future as a screening device in diagnosis.

• 2013-04-25

著者

• Cho Won-ju

  Department Of Electronic Material Engineering Kwangwoon University

• Kwon Jae-young

  Department Of Orthopaedic Surgery Seoul St Mary's Hospital

• Jang Hyun-June

  Department of Electronic Materials Engineering, Kwangwoon University, Seoul 139-701, Korea

• Bae Tae-Eon

  Department of Electronic Materials Engineering, Kwangwoon University, Seoul 139-701, Korea

• Lau Hui-Chong

  Biomedical Research Institute, Kyungpook National University, Daegu 700-721, Korea

• Lim Jeong-Ok

  Biomedical Research Institute, Kyungpook National University, Daegu 700-721, Korea

• Kwon Jae-Young

  Department of Biological Science, Sungkyunkwan University, Suwon, Gyeonggi 440-746, Korea

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