Synthesis of <I>N</I>-Terminal Nonapeptide of Trypsinogen and Its Hydrolysis by Trypsin
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概要
- 論文の詳細を見る
An <I>N</I>-terminal nonapeptide fragment of bovine trypsinogen, H-L-Val-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-L-Ile-L-Val-Gly-OH, was synthesized <I>via</I> two routes and its susceptibility with trypsin was investigated. Protected derivatives, <I>t</I>-butyloxycarbonyl protected nonapeptide <I>t</I>-butyl ester and benzyloxycarbonyl protected nonapeptide benzyl ester, were prepared by stepwise elongation. Removal of the protecting groups was carried out by treatment with trifluoroacetic acid and hydro-genation respectively. The naked peptide was purified with chromatography on ECTEOLA cellulose column using dilute acetic acid as a solvent. Upon lyophilization the desired nonapeptide was obtained as colorless powder, a portion of the product being digested with trypsin. Rate of tryptic hydrolysis of the peptide was found to be unexpectedly low in the absence of calcium ion, while considerable acceleration of the hydrolysis by calcium ion was noted.
- 公益社団法人 日本化学会の論文
著者
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Izumiya Nobuo
Laboratory Of Biochemistry Faculty Of Engineering Kyushu Sangyo University
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KATO Tetsuo
Laboratory of Biochemistry, Faculty of Science, Kyushu University
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Yoshida Norio
Laboratory of Biochemistry, Faculty of Science, Kyushu University
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