血液中の中性β-N-アセルチルグルコサミニダ-ゼ活性の測定による凍結・解凍魚と未凍結魚との鑑別〔英文〕

概要

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A method for distinguishing frozen-thawed from unfrozen fish was developed. The principle of this method is based on the findings that in the blood of most species of fish, particularly in the cytosol of the red blood cells, the presence of a neutral β-N-acetylglucosaminidase which does not express activity in the intact cells unless the cells are damaged, e. g. by freezing and thawing. The differentiation was performed by fluorometric determination of the neutral β-N-acetylglucosamini-dase activity in fish blood. For the fresh and for the chilling fish kept for one to three days at 4°C, the blood showed little fluorescence, while for the frozen fish stored at -20°C or -40°C even for one day, the blood showed a strong fluorescence. The method is applicable to most commercial fish species, both marine and freshwater. Compaed with other available enzymatic methods, the method is simple and reliable. The entire procedure can be done in about 20 min.