シアル酸含有オリゴ糖の遊離 (鯨鼻軟骨ケラト硫酸の酵素分解-4,5-〔英文〕)

概要

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The present work was undertaken to examine the fine structure, [especially the linkage region of sialic acid], of whale cartilage keratosulfate using the keratosulfate-degrading enzyme from an enteric bacterium. After the keratosulfate was digested with the enzyme, some oligosaccharides produced were isolated by means of Sephadex filtration and paper electrophoresis, and subjected to chemical analysis. The result showed that two types of oligosaccharides were produced by the enzyme: one of them was a group of di- and tetrasaccharides containing in an equimolar ratio N-acetylglucosamine, galactose and sulfate, and the other was a tetrasaccharide containing two molecules of galactose and one molecule each of N-acetylglucosamine, sulfate and sialic acid. Judging from the results of chemical analysis of this tetrasaccharide and of enzymatic treatment with glycosidase such as β-galactosidase, β-N-acetylglucosaminidase and neuraminidase, the sugar sequence of the saccharide was decided as N-acetylneuraminic acid-galactose-N-acetylglucosamine(sulfate)-galactose-**. Thus, the sialic acid was demonstrated to be attached directly to the nonreducing end of the keratosulfate.
日本水産學會の論文