凍結・解凍魚と未凍結魚との鑑別に用いる簡便法と試験紙法の開発〔英文〕

概要

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We developed a simple routine method and test paper method for differentiating frozen-thawed fish from unfrozen one. The suggested procedure consists of two steps; (i) incubation of a drop of fish blood with a fluorescent substrate in a Durham tube for 10 min at room temperature, (ii) ter-mination of the reaction by adding an alkaline buffer and observing on the color change of the blood in Durham tube under a UV-lamp. For unfrozen fish, the blood shows red while it shows blue for frozen-thawed fish. The method requires only 10μl of the blood and about 15 min for the entire procedure. Based on the routine method, a test paper method was developed. The differentiation can be performed by smearing a drop ofthe blood on the test paper, and after keeping it at room temperature for 10 min, placing a drop of the alkaline buffer on the paper, and then observing the color change under a UV-lamp. Both the routine and test paper methods are ap-plicable for testing the most common edible fish.