魚病菌Vibrio anguillarumに対するPseudomonas sp.の溶菌および致死作用

概要

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A Pseudomonas sp. (L 1-7) capable of lysing Vibrio anguillarum, important pathogenic organism of cultured fish, was isolated from pond water for fish culture and tank water for plankton culture. The high yield of lytic factor in the supernatant of strain L 1 culture was obtained by shaking culture at 25°C for 24 hours in the medium containing 1% meat extract, 0.5% polypepton, 0.4% yeast extract and 0.3% glucose at pH 7.0. The activity of the lytic factor was highest at around 45°C and a little lower at 30°C; it was inactivated by heating at 50°C for 4 hours or at 60°C for 15 minutes. Lytic factor produced by strain L 1-7 was found to show varying degree of activity against various gram negative organisms other than V. anguillarum, depending upon the difference in strains. Gram positive organisms such as Bacillus subtilis and the other Bacillus organisms were lysed more or less, whereas Streptococcus faecalis and S. faecium strains were completely insensitive to the lytic factor. It is then considered that no correlation exists between the lytic action of the organisms and gram reaction of the organisms examined. The culture supernatant of strain L 1 was not only able to lyse the V. anguillarum cells, but also found to show remarkable lethal action.
日本水産學會の論文