ニジマス肝臓のβ-ガラクトシダーゼに関する研究-2-各種化合物の影響

概要

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The effects of sugars, lactones, heavy metals, and salts on the β-galactosidase isolated from the liver of rainbow trout were investigated. 1. The β-galactosidase I and II were activated by glucose, sucrose, gluconolactone, and glucarolactone. 2. Galactose inhibited both isozymes competitively, while galactonolactone inhibited the enzyme I competitively and the enzyme II noncompetitively. 3. Lactose, a β-galactoside, was not only resistant but inhibitory to β-galactosidase I and II of the rainbow trout. 4. Heavy metal ions inhibited both isozymes noncompetitively. The metal inhibition was recovered by the addition of cysteine, but not by EDTA. 5. The β-galactosidase I and II of the rainbow trout were found to be activated by Cl-, while the bacterial β-galactosidase was reported to be inhibited by Cl- and activated by Na+. Both the substrate affinity and Vmax. of the enzyme I were increased by NaCl which caused only the elevation of the substrate affinity of the enzyme II.
社団法人日本水産学会の論文