コイおよびニジマス肝臓のピルビン酸キナーゼの特徴

概要

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The pyruvate kinase(EC 2.7.1.40, PK)from carp hepatopancreas and rainbow trout liver was purified and characterized. It was assured, by chromatography on DEAE-Sephadex A-50 column, that there are two forms of PK in both fish livers. The major components of PK from carp and trout showed the maximum activities at pH 6.38 and 6.04, respectively. The molecular weight of PK from both species as estimated by gel filtration was 234, 000. Both enzymes were activated by FBP and inhibited by ATP, Ca2+ and low level of Cu2+. Several divalent cation (Mg2+or Mn2+) and monovalent cation(K+ or NH4+) were absolutely essential for their activities. Theresult of kinetic investigation suggested that the reaction of carp PK was due to “sequential” mechanism, while that of rainbow trout enzyme was due to “Ping Pong” mechanism.
公益社団法人日本水産学会の論文