魚類の血清と胆汁によるβ-グルクロニダーゼの阻害

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The inhibitory effects of the biological fluids such as serum, urine, or bile have been reported by many investigators. DOHRMANN et al.9) attributed the inhibitory effect of human serum to α-and β-globulins, and MATSUSHIRO7) suggested that effect of human bile is due to glucarolactone. We investigated the inhibitory effects of fish serum and bile on liver β-glucuronidase of rainbow trout as well as on bovine liver enzyme. As shown in Fig. 1, β-glucuronidase of bovine liver is 81 and 93% inhibited by fresh bovine and trout sera, respectively, Trout liver enzyme is inhibited to the extent of 90 and 87% by fresh trout and bovine sera (Table 1). The dialysate of the serum scarcely inhibited the enzyme at all, while the non-dialysable fraction retained the effect. The methanol extracts of the bovine serum showed only a negligible effect. The inhibition of the enzyme by bile is much more pronounced than that by sera. As illustrated in Fig. 2, even such dilute solution as 0.5% shows considerable inhibitory effect. The influence of dialysis on the inhibitory effect of bile differs from that on sera. The dialysable fraction of the carp bile apparently shows an inhibitory effect, although it is less than that of the non-dialysable fraction (Table 2). Several components which show the inhibitory effect have been obtained by gel filtration with Sephadex G-25 (Fig. 3). However, none of them shows competitive inhibition which is characteristic to glucarolactone (Fig. 4).
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魚類の血清と胆汁によるβ-グルクロニダーゼの阻害

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