飲食品の汚染指標としての腸球菌に関する研究-VI : 腸球菌と他の菌種との色素に対する感受性の比較

概要

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The sensibilities of enterococci and other organisms to various dyes or sodium azide added in citrate yeast dextrose agar were mutually compared. Eight kinds of triphenylmethane dyes, five kinds of acridine dyes and methylene blue were used in this study. Gram-negative organisms were found to be more resistant to triphenylmethane dyes than gram-positive ones. As a whole, among the gram-positive organisms so far examined, enterococci showed the highest resistance to these triphenylmethane dyes. Enterococci were clearly distinguished from other gram-positive organisms in their particularity of high resistance to ethyl violet and crystal violet. As to the sensibility to acridine dyes or methylene blue, no appreciable difference was found between different groups of organisms. On the other hand, it was shown that enterococci were characterized by their high resistance to sodium azide among all gram-negative and gram-positive organisms examined. A combination of sodium azide and ethyl violet as inhibitory agent, as presented by LITSKY and others, may be most suitable to be used in the medium for selective cultivation of enterococci.