BCG経口接種モルモットの抗結核免疫力

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Adult guinea pigs weighing 350-400 g were inoculated orally with freeze-dried BCG vaccine in amount of 80 mg as one dose resuspended in physiological saline inserting a specially provided inoculation instrument deeply and quickly into oesophagus. Thereafter, body weight increase, tuberculin reactions and the fate of BCG were investigated in the 1st experiment. In all animals, body weight increased fairly well and tuberculin reactions converted to positive as early as at the 4th week after inoculation. The viable bacilli recovered from organs revealed that BCG absorbed through Peyers patches distributed rather quickly to the remote organs such as bronchial lymph nodes representing large number of viable units accompanying marked swelling of lymph nodes in earlier stage after inoculation.<BR>In the 2nd experiment, animals were divided into 4 groups of 6 guinea pigs each. The 1st group was inoculated with living BCG, the 2nd with heat-killed BCG, the 3rd with heat killed BCG in Freunds incomplete adjuvant(F. I. C. A.), respectively, and the 4th was left as untreated control. The former 3 groups were inoculated orally either with living or heat killed BCG of the same concentration as living BCG twice at 3 weeks interval. Three weeks after the 2nd oral inoculation, i.e. at the 9th week when all animals of the 1st group showed positive tuberculin reactions, while those of the remaining 3 groups were negative except an animal each of the 2nd and the 3rd groups, all animals were infected subcutaneously with H37Rv strain. After additional 3 weeks, they became strongly positive to tuberculin. Then, 3 animals each were sacrificed at the 3rd and the 6th week after infection. The macroscopic findings and the number of virulent tubercle bacilli recovered from organs revealed that the animals of the 1st group alone were affected obviously slighter than those of the remaining 3 groups. Among the latter 3 groups, no statistically significant difference existed. Serological test revealed that only in one animal of the 3rd group, which was positive to tuberculin, xhaemoagglutination titer was demonstrated as high as 32 fold.<BR>The present study showed clearly that even the adult guinea pigs could be sensitized fairly well to tuberculin and immunized against virulent tubercle bacilli infection by the oral administration of BCG vaccine, provided that the vaccine of high viability is inoculated into empty stomach.

BCG経口接種モルモットの抗結核免疫力

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