無細胞半合成培地を用いる鼠癩菌分離培養の試み

概要

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A biological significance was suggested to be shown by an acid-fastness map devised from investigation of the effect of ultraviolet irradiation on the acid-fastness of mycobacteria. Then, attempts were made to cultivate Mycobacterium lepraemurium Hawaii from nodules of infected mice in a cell-free, semi-synthetic soft agar medium. The basic composition of this medium was modified to some extent from that used for the successul cultivatioh of M. paratuberculosis Johnei.Experiments were carried out repeatedly five times. Those organisms which had multiplied even to a limited extent were observed to form microcolony-like masses, which were composed of elongated, strongly acid-fast rods. It was interesting to note that when a medium contained both glycerol and glucose as carbon sources in addition to ferricion, it seemed to support the growth of M. lepraemurium to some extent.
日本細菌学会の論文