牛型菌の発育に及ぼす2, 3の炭素源の影響

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To stimulate the growth of dysgonic bovine strains of mycobacteria, the effect of theaddition of Tween 80 to M-Y RP medium was investigated, which contained glucose and Napyruvate as main carbon sources and was regarded as the best medium among those tested inthe foregoing experiments. In any of the media, which contained only one of the abovementioned three carbon sources, i. e. glucose, Na pyruvate and Tween 80, independently, nofavorable growth was observed. However, in the media, which contained Na pyruvate andTween 80, obviously better growth was obtained in most of the strains examined than in M-YRP medium. On the other hand, the growth was stimulated obviously by the addition of Napyruvate to the medium, which contained glucose and Tween 80 but did not enhance thegrowth of organisms favorably. Therefore, for the better utilization of Na pyruvate andTween 80 as carbon sources, it seemed better to remove or reduce the amount of glucose toless than 0.5%. The semi-synthetic agar medium of the following prescription seemed to beadequate, at present, to stimulate the growth of dysgonic bovine strains of mycobacteria: KH2PO4 2.0, Na2HPO4.12 1120 1.5, Asparagine 6.0, Na. citrate 2.0, CaCl2 0.0025, MgSO4.7 H2O 0.02, Glucose 5.0, Na. pyruvate 2.0, Tween 80 0.1ml, BSA Fraction V 5.0, Yeast RNA100μg, Agar-agar 15.0, Aq. 1, 000 ml.
日本結核病学会の論文