無細胞・半合成・半流動寒天培地を用いる人癩菌の培養 : II. 培地組成,前処理および培養温度の検討

概要

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Bacterial suspensions prepared from the nodules of 3 cases of L-type patients were inoculated into 0.1% semi-liquid agar media of various base compositions for the primary isolation. Prior to the cultivation, the bacterial suspension was mixed well with an equal volume of 2% NaOH at 37°C for 60 minutes to eliminate contamination, and was neutralized with N-HCl to adjust its pH to 6.6∼6.8. In any of the media used, obvious increase was observed in number and size of bacterial masses, showing fairly strong resistance to the alkali treatment. It was revealed again that this organism could be cultivated in cell-free, semi-synthetic softagar (0.1%) media, and that the growth seemed to be better in the medium which contained both glycerol and glucose as carbon sources than those which contained glucose alone. With regard to the incubation temperature, it was demonstrated clearly that the adequate temperature for the growth was 37°C, and not 30°C or less.
日本細菌学会の論文