ツベルクリン反応に及ぼすTween80添加の影響-2-人体におけるTween80添加PPDsによる反応

概要

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In the present studies the effect of Tween 80 both on the tuberculin reaction in human subjects and on the protection against the drop of potency by preservation were investigated. Samples tested were the PPDs solutions in phosphate buffer with or withou t addition of Tween 80 and the phosphate buffer solution with Tween 80. Human subjects tested were the school children from 6 to 14 years old, to whom these samples were injected intradermally. Results: 1) Non-specific reaction due to the control, phosphate buffer, solution with Tween 80 appeared in a low percentage as similar degree as the other kinds of control solution. 2) In the same PPDs doses, erythema appeared obviously larger by the PPDs add e d with Tween 80 than that of without, but the rate of palpable induration was not markedly different between them. Frequency distribution curves of erythema size according to the various doses of PPDs revealed that by PPDs without Tween 80 the distribution curves shifted to the smaller erythema size proportionally according to the decrease of PPDs doses, whereas by PPDs with Tween 80 such a shift proportional to the dosage was not observed. 3) Marked drop was noticed in the potency of PPDs s o lution added with Tween 80 after about 2 months' preservation and transportation to a long distance at the temperature of about 20°C to 25°C. From these results, the addition of Tween 80 to PPDs solution to keep its potency unchanged seemed to be undesirable.