DILUTING SOLUTION FOR THE OLD TUBERCULIN

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Since 1947 boric acid-borax buffer has been used in Japan as the buffer solution to dilute old tuberculin. However, erythema due to non-specific reaction was noticed not seldom by the use of the tuberculin diluted with this buffer. Then, the comparative studies were conducted. on the appearance of non-specific reaction and the preservability of the potency when various. kinds of diluting solution were employed. In these studies the inhabitants of Amami main island were examined, where no tuberculin injection has previously been made. The diluting solutions compared were boric acidborax. buffer, phosphate buffer and physiological saline, and the old tuberculin was diluted by them. into 1: 2, 000, respectively. The resulting dilutions were compared of their potency not only immediately after preparation but also after about 2 years preservation either in a refrigerator or in an incubator. Results: 1) Erythema due to non-specific reaction appeared obviously more frequently by boric acid-borax buffer than by phosphate buffer. Quite the same trend was observed by the old. tuberculins diluted with above mentioned buffer solutions. 2) After 2 years preservation in a refrigerator no decrease was noticed in the potency of both tuberculins diluted with either boric acid-borax buffer or phosphate buffer, while the potency of tuberculin diluted with physiological saline alone decreased markedly. Preservation in an incubator resulted in the decrease of potency of all of the tuberculin solutions prepared with the above mentioned 3 kinds of diluting solutions. From these results, it became clear that phosphate buffer and physiological saline are preferable to minimise non-specific reaction, and boric acid-borax buffer and phosphate buffer, on the other hand, are preferable to keep the potency of diluted old tuberculin unchanged for a rather long time period. As the conclusion, phosphate buffer seemed to be most adequate as the diluting solution of old tuberculin.
一般社団法人日本結核病学会の論文

DILUTING SOLUTION FOR THE OLD TUBERCULIN

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