抗酸菌の溶原化に関する研究 : 第3報溶原化された抗酸菌のMitomycin Cなどによるフアージ誘発

概要

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A lysogenized Mycobacterium, strain F 21 (LI), was successfully induced by mitomycin C. The induction rate was maximum at 10 minutes after the addition of mitomycin C at a concentration of 1μg/ml. However, the induction rate was relatively lower than that of E. coli K 12 (λ) reported by Otsuji et al. Burts size of induced F 21 (LI) was calculated as 14.5.Two strains of Mycobacteria, F 21 and Jucho, both belongs to Mycobacterium smegmatis from their phage patterns, were lysogenized by four strains of temperate phage, LI, Y 13L, Y 13S and A 6, respectively, and each of them was treated with mytomycin C. The results obtained, revealed that in Mycobacteria as well as in other bacterial species, induction might depend upon the phage strain rather than bacterial strain which was lysogenized by the phage.Mitomycin C resistant F 21, lysogenized by the phage LI, could not be induced by mytomycin C but it could be induced by ultraviolet irradiation.Nitromin, which is known as an inducing agent, could not induce the phage LI from the F 21 (LI) under the present experimental conditions.
日本細菌学会の論文