肝組織血流量及び組織酸素分圧同時測定による肝微小循環の解析

概要

論文の詳細を見る
We devised a new system for simultaneous monitoring of tissue perfusion and oxygenation of the same lobe of rat liver. The tissue perfusion was monitored by measuring the clearance of electrochemically generated hydrogen, and calculating its blood flow. The tissue oxygenation was monitored by measuring the tissue oxygen tension using a polarographic microelectrode.This system was then applied to investigate the effects of intravenously infused liver extract and FAD on hepatic perfusion and oxygenation in the rat. Normal saline was used as control. When normal saline was infused, the mean blood flow (under the constant generating current of 5μA, 20sec) and the mean oxygen tension were 47.4ml/min/100g and 18.1mmHg, respectively. The infusion of liver extract increased both the blood flow and oxygen tension, whereas only oxygen tension increased when FAD was infused. In both cases, the increase of oxygen tension was statistically significant when compared with that of normal saline.The analysis using this system is useful in obtaining the indices of hepatic microcirculation, and it can be applied to study the effects of drugs on liver.
財団法人日本消化器病学会の論文