Studies on Immunoreactive Gastrin in Tissue of Human Digestive System

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Immunoreactive gastrin (IRG) was determined by double antibody technique mainly in gastrointestinal tissues and in the other various tissues obtained at autopsy, as well as by biopsy operating under endoscopy.The largest amount of IRG (4, 937ng/g(w/w)) was found in the antral mucosa among tissues tested, and the concentrations in the gastrointestinal tissues decreased following the distal portion, showing an amount of 3.2ng/g in the ileum. It was noted that the mean IRG level in the antral mucosa of cases accompanying with active gastroduodenal ulcer at autopsy was relatively lower as 617ng/g(w/w), and an abundant amounts of IRG were found in biopsy specimens not only of the antral mucosa (345.5ng/mg) but of the corporal mucosa (17.4ng/mg) of the stomach from a patient with pernicious anemia.IRG in boiled extracts of gastrointestinal mucosa was fractionated by use of Sephadex G-50 gel filtration and starch gel electrophoresis. Most of IRG (more than 90%) in the antral mucosa was separated in region of little G (LG), and big G (BG) showed an increase following distal portion of the duodenum. It was, however, interesting that a little amount of big big G was detected in gastric mucosa from a patient with pernicious anemia.Summarizing the results, production, storage and release mechanism of gastrin was discussed.
財団法人日本消化器病学会の論文