FOLLICLE-STIMULATING HORMONE-RELEASING FACTOR (FSH-RF) AND LUTEINIZING HORMONE-RELEASING FACTOR (LH-RF) IN RAT HYPOTHALAMIC EXTRACT
スポンサーリンク
概要
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Hypothalamic tissues of female rats were extracted with 1.0<I>M</I> acetic acid. The acidic extract was lyophilized and subjected to gel filtration on Sephadex column and assayed for follicle-stimulating hormone-releasing factor (FSH-RF) activity and luteinizing hormone-releasing factor (LH-RF) activity by estimating FSH and LH using the organ culture method of rat anterior pituitaries. FSH activity was measured by the method of Steelman-Pohley, and LH activity by the method of Parlow. Gel filtration on a short column of Sephadex G-25 in 0.1M pyridine acetate provided a single fraction which showed significant FSH-RF and LH-RF activity. Each activity showed a doserelated increase. Application of a long column of Sephadex G-25 in 0.05M pyridine acetate brought about separation of a peak for FSH-RF activity from that for LH-RF activity. The former activity was eluted from the column just before the appearance of the latter. These results indicated that the secretion of FSH and LH from the rat anterior pituitary was regulated by the specific hypothalamic FSH-RF and LH-RF, respectively.
- 社団法人 日本内分泌学会の論文
著者
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WATANABE TAKU
Department of Obstetrics and Gynecology, School of Medicine University of Tokyo
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Ichikawa Hisashi
Department Of Chemistry The University Of Tokyo
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Mizuno Masahiko
Department Of Neurosurgery And Pediatrics Kansai Medical School
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Kigawa Tomonori
Department Of Obstetrics And Gynecology Faculty Of Medicine University Of Tokyo Tokyo
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Kobayashi Takuro
Department Of Dermatology Field Of Sensory Organology Kagoshima University Graduate School Of Medica
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KOBAYASHI Takashi
Department of Applied Physics,Faculty of Engineering,Nagoya University
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AMENOMORI YOSHIHIKO
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo
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