Increase of Calmodulin III Gene Expression by μ-Opioid Receptor Stimulation in PC12 Cells
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概要
- 論文の詳細を見る
Calmodulin (CaM) is a principal multifunctional mediator of Ca2+ signaling in cells. It is reported that morphine increases CaM contents in mouse brain. However, the precise mechanism of CaM induction by morphine is unknown. We investigated the changes of CaM by opioid receptor stimulation in mRNA and protein levels. Expression of CaM was increased in dose- and time-dependent manners by morphine with RT-PCR assay in PC12 cells, and naloxone inhibited the effect of morphine. The expression was also increased with DAMGO (μ-opioid agonist), but not by DPDPE (δ) and U50488 (κ). Northern blot analysis revealed that the CaMIII gene was responsive to morphine or DAMGO. CaM protein increased by DAMGO were distributed in both soluble and membranous fractions in the cells. Taken together, the data suggest that morphine induces the expression of CaMIII gene through μ-opioid receptor stimulation.
- 社団法人 日本薬理学会の論文
- 2000-12-01
著者
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MIKI Naomasa
Department of Pharmacology, Osaka University Medical School
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Miki Naomasa
Department Of Pharmacology Osaka University Medical School
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Kuo Che-hui
Department Of Pharmacology Osaka University Medical School
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ICHIKAWA Hisashi
Department of Obstetrics and Gynecology, Sekishinndo Hospital
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Miki Naomasa
Department Of Pharmacology I Osaka University School Of Medicine
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NIU Sanyoung
Department of Pharmacology, Osaka University Medical School
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GAN Yehua
Department of Pharmacology, Osaka University Medical School
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NISHIKAWA Etsuko
Department of Pharmacology, Osaka University Medical School
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SADAKATA Tetsushi
Department of Pharmacology, Osaka University Medical School
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Ichikawa Hisashi
Department Of Chemistry The University Of Tokyo
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Ichikawa Hisashi
Department Of Pharmacology Osaka University Medical School
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Gan Yehua
Department Of Pharmacology Osaka University Medical School
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Niu Sanyoung
Department Of Pharmacology Osaka University Medical School
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Nishikawa Etsuko
Department Of Pharmacology Osaka University Medical School
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Sadakata Tetsushi
Department Of Pharmacology Osaka University Medical School
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KUO Che-Hui
Department of Pharmacology I, Osaka University School of Medicine
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