Inhibition by Naloxone of Promoter Activity of the Neurofilament Gene in SK-N-SH Cells
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概要
- 論文の詳細を見る
Chronic administration of morphine is known to decrease the levels of neurofilaments(NFs)in the ventral tegmental area.We ligated a promoter region of the mouse 68−KDa neurofilament(NF−68)gene to the pGL3−enhancer vector containing a luciferase gene, transfected it into SK−N−SH cells and then analyzed transcriptional activity in the cells treated with agonists or antagonists of opiate receptors.The activity of the NF−68 promoter was suppressed by naloxone about 55% at 10-5 M and 30% at 10-7 M at 48h, but suppressed not by morphine.Naltrexone at 10-5 M suppressed the promoter activity about 20%, but levallorphan, DAMGO, DPDPE and U50488 did not.The inhibition by naloxone was dose−dependent and not reversed by morphine.The inhibitory effect of naloxone was not observed in N18TG−2 cells and PC12 cells.Experiments with various deletion mutants revealed that a region responsible for naloxone suppression spans from −328 to − 101 in the gene.These results suggest that naloxone has the ability to suppress transcriptional activity in some neurons.
- 社団法人 日本薬理学会の論文
- 2000-01-01
著者
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Miki N
Osaka Univ. Osaka Jpn
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MIKI Naomasa
Department of Pharmacology, Osaka University Medical School
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KUO Che-Hui
Department of Pharmacology, Osaka University Medical School
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NIU San-Yong
Department of Pharmacology, Osaka University School of Medicine
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TAIRA Eiichi
Department of Pharmacology, Osaka University School of Medicine
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MURAOKA Osamu
Department of Pharmacology, Osaka University School of Medicine
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IRIE Yasuyuki
Department of Pharmacology, Osaka University School of Medicine
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GAN Ye-Hua
Department of Pharmacology, Osaka University School of Medicine
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DO Eunju
Department of Pharmacology, Osaka University School of Medicine
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Miki N
Department Of Pharmacology Osaka University School Of Medicine
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Muraoka Osamu
Department Of Pharmacology Osaka University School Of Medicine
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Niu San-yong
Department Of Pharmacology Osaka University School Of Medicine
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Kuo C‐h
Department Of Pharmacology Osaka University School Of Medicine
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Kuo Che-hui
Department Of Pharmacology Osaka University Medical School
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Gan Ye-hua
Department Of Pharmacology Osaka University School Of Medicine
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Taira Eiichi
Department Of Pharmacology Osaka University School Of Medicine
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Taira Eiichi
Department Of Pharmacology Osaka University Graduate School Of Medicine
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Do Eunju
Department Of Pharmacology Osaka University School Of Medicine
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Irie Y
Department Of Pharmacology Osaka University School Of Medicine
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Miki Naomasa
Department Of Pharmacology I Osaka University School Of Medicine
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Irie Yasuyuki
Department Of Pharmacology Osaka University School Of Medicine
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Muraoka Osamu
Department Of Molecular Oncology Biomediacl Research Center Osaka University Medical School
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KUO Che-Hui
Department of Pharmacology I, Osaka University School of Medicine
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