血清中胆汁酸の酵素免疫学的測定法

概要

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A sensitive, specific and conventional enzyme immunoassay (EIA) for the measurement of ursodeoxycholic acid (UDCA) in human serum was described. Anti-UDCA serum was raised in rabbit using UDCA-bovine serum albumin conjugate as antigen. UDCA-alkaline phosphatase(calf intestine) conjugate prepared by a method of carbodilmide was used as a tracer. The assay was carried out by the two antibody method, and alkallne phosphatase activity of bound complex was measured by King-King method. The ratio of bound to total enzyme activity decreased linearly with a logarithmic increase in ursodeoxycholic acid concentration from 20 to 900p moles.<BR>The specificity and sensitivity of this EIA were similar to those of RIA reported previously. The serum UDCA concentrations measured by this EIA were highly correlated with those by gaschromatography or RIA.<BR>It is concluded that this UDCA-EIA system is a useful tool for routine analysis of serum bile acids in the clinical fields.
社団法人日本肝臓学会の論文