D環抱合型エストロゲンの高速液体クロマトグラフィーによる一斉分離検出 (<特集> バイオサイエンスと分析化学)

概要

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The analytical method of ring D-conjugated estrogens, the existence of which in pregnancy urine is known or estimated, was established by using HPLC. Eleven kinds of these conjugates were satisfactorily separated by using octadodecylsilica as the stationary phase and a mixture (60 : 40) of 0.5% NH_4H_2PO_4(pH 3.0) and methanol as the mobile phase and detected sensitively by using electrochemical detector (ECD). Conjugates having a catechol or guaiacol structure could be separately determined from those having a phenolic structure by controlling the detector voltage. The detection limit (S/N=3) of the catechol, guaiacol and phenolic conjugates were about 2,100 and 50pg, respectively.
社団法人日本分析化学会の論文
1995-10-05