Inhibition by chlorpromazine, metals and I-ascorbic acid of calcium-ATPase and magnesium-ATPase in bovine adrenal medullary microsomes.
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概要
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Effects of chlorpromazine, metals and I-ascorbic acid (AA) on Ca<SUP>2+</SUP>-ATPase and Mg<SUP>2+</SUP>-ATPase in microsomal and granular fractions obtained from the bovine adrenal medulla were studied. Marker enzyme analysis on microsomal subfractions in a discontinuous sucrose density gradient showed a correlation of distribution between ATPase activities and plasma membrane. The two ATPase activities in such plasma membrane-rich microsomes were reduced by chlorpromazine, Hg<SUP>2+</SUP> and Cu<SUP>2+</SUP> (0.3 mM of each), and their effects were greater on the Mg<SUP>2+</SUP>-ATPase activity. Zn<SUP>2+</SUP>(0.3 mM) also reduced only the Mg<SUP>2+</SUP>-ATPase activity. AA (3 mM) reduced the two ATPase activities to an equal extent. Nevertheless, the inhibitions of ATPases by Hg<SUP>2+</SUP>, Cu<SUP>2+</SUP> and Zn<SUP>2+</SUP> were decreased, unaltered and additively enhanced in combination with AA, respectively. We also observed high Mg<SUP>2+</SUP>-ATPase activity in the granule-rich fraction, but this ATPase activity was unaffected by all of the above agents. These results indicate that Mg<SUP>2+</SUP>-ATPase in the plasma membrane-rich microsome of adrenal medulla is inhibited by chlorpromazine, Hg<SUP>2+</SUP>, Cu<SUP>2+</SUP> and Zn<SUP>2+</SUP> more significantly than Ca<SUP>2+</SUP>-ATPase, but Mg<SUP>2+</SUP>-ATPase in the granular fraction is unaffected, and that AA changes the potency of inhibition by some metals of ATPases diversely.
- 公益社団法人 日本薬理学会の論文
著者
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Yamada Shizuo
Department Of Biophamacy School Of Pharmaceutical Sciences University Of Shizuoka
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Hayashi Eiichi
Department Of Pharmacology Shizuoka College Of Pharmaceutical Sciences
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Hayashi Satoru
Department Of Dermatology Faculty Of Medicine University Of Yamanashi
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YAMANAKA Kyozo
Department of Pharmacology, Fukui Medical School
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