Effects of Prostaglandin E1 and Dipyridamole on Disposal of Colloidal Carbon via Glomerular Mesangial Channels

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We investigated the effects of prostaglandin E1 (PGE1) and dipyridamole on the disposal of macromolecules via mesangial channels to understand the antinephritic effect of PGE1. To increase glomerular carbon, rats were injected i.v. first with anti-glomerular basement membrane (GBM) serum and then with 30 mg/100 g body weight of carbon. Carbon in the glomerulus was detected with an image analyzer. Administration of anti-GBM serum resulted in about a 2 to 3-fold increase in glomerular uptake of carbon in the normal rats. To investigate the day-to-day situation of glomerular carbon, the right kidney was removed the day after carbon injection, and the left kidney was isolated on days 7, 14 and 21. By day 21, glomerular carbon had decreased significantly. Rats with carbon received PGE1 0.1×2 and 1.0×2 mg/kg, s.c., and dipyridamole 200 and 400 mg/kg, p.o., for 10 days after the isolation of the right kidney. PGE1 administration did not cause a decrease in the amount of glomerular carbon compared with that of the control. However, the dipyridamole group showed a more rapid decrease of glomerular carbon. It is concluded that PGE1 has no effect on the mesanigial channels, but dipyridamole can accelerate the disposal of glomerular carbon by way of the mesangial channels.