Expression of the Nucleocapsid Protein Gene of the Canine Distemper Virus.
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概要
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We constructed a cDNA clone of canine distemper virus (CDV) encoding an entire nucleoprotein (NP) gene, by means of the reverse transcription-polymerase chain reaction (RT-PCR). The cloned NP gene was inserted into the eucaryotic expression vector, pRVSV. After transfection of the plasmid into Vero cells, we examined the expression of CDV-specific NP antigen by means of indirect immunofluorescence assay (IFA) and Western blotting, using various antibodies against NP of CDV and an antiserum against NP of measles virus. The CDV-NP specific antigen was detected in the nuclei of the cells transfected with pRV-ON, by means of IFA with antibodies specific to the NP.
- 公益社団法人 日本獣医学会の論文
著者
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Kai Chieko
Department Of Veterinary Microbiology Faculty Of Agriculture The University Of Tokyo
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Shin Yeon-sil
Department Of Viral Infections Research Institute For Microbial Diseases Osaka University
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Tomonaga Keizo
Department Of Veterinary Microbiology The University Of Tokyo
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Mori Takeshi
Department Of Applied Chemistry Graduate School Of Engineering Kyushu University
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MIKAMI Takeshi
Department of Cardiovasology, Surugadai Nihon University Hospital
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TOMONAGA Keizo
Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo
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IWATSUKI Kiyoko
Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo
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Mori Takeshi
Department of Applied Chemistry and Chemical Engineering
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SHIN Yeon-Sil
Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo
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