Construction of a Bacterial Artificial Chromosome Library for a Myxobacterium of the Genus Cystobacter and Characterization of an Antibiotic Biosynthetic Gene Cluster

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概要

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• A bacterial artificial chromosome (BAC) library was constructed to isolate the biosynthetic gene cluster for the polyketide/peptide hybrid-type antibiotic cystothiazole A from the myxobacterium Cystobacter fuscus strain AJ-13278. Sequence analysis of a 63.9 kb contiguous region that encompasses the biosynthetic gene cluster (cta) led to the identification of a polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) hybrid gene cluster 32.1 kb in size, which consists of six open reading frames (ORFs), ctaB to ctaG, as well as downstream genes ctaJ and ctaK (1.0 and 0.9 kb, respectively) responsible for the final biosynthetic steps. The genes ctaB, ctaE, and ctaF encode PKSs, the genes ctaC and ctaG encode NRPSs, and ctaD encodes an NRPS-PKS hybrid enzyme. Disruption of ctaD impaired cystothiazole A production. Additionally, two downstream genes, ctaJ and ctaK, which encode a nitrilase and an O-methyltransferase, respectively, must be responsible for the final methyl ester formation in the cystothiazole A biosynthesis.

• 社団法人 日本農芸化学会の論文
• 2005-07-23

著者

• Suzuki Y

  Exploratory Medicinal Sciences Pgrd Nagoya Laboratories Pfizer Pharmaceuticals Inc.

• Suzuki Yoshihiro

  Graduate School Of Bioagricultural Sciences Nagoya University

• Sakagami Y

  Nagoya Univ. Nagoya Jpn

• Sakagami Youji

  Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Sakagami Youji

  Graduate School Of Bio-agricultural Sciences Nagoya University

• Okada Masahiro

  Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.

• QI Jianhua

  Graduate School of Bioagricultural Sciences, Nagoya University

• OJIKA Makoto

  Graduate School of Bioagricultural Sciences, Nagoya University

• Qi Jianhua

  Graduate School Of Bioagricultural Sciences Nagoya University

• Suzuki Yumiko

  Exploratory Medicinal Sciences Pgrd Nagoya Laboratories Pfizer Pharmaceuticals Inc.

• FENG Zhiyang

  Graduate School of Bioagricultural Sciences, Nagoya University

• TSUGE Takashi

  Graduate School of Bioagricultural Sciences, Nagoya University

• OBA Yuichi

  Graduate School of Bioagricultural Sciences, Nagoya University

• KOBAYASHI Tetsuo

  Graduate School of Bioagricultural Sciences, Nagoya University

• Okada Masahiro

  Dep. Of Chemistry Tohoku Univ.

• Oba Yuichi

  Graduate School Of Bioagricultural Sciences Nagoya University

• Oba Yuichi

  Graduate School Of Bioagricultural Sciences

• Feng Zhiyang

  Graduate School Of Bioagricultural Sciences Nagoya University

• Ojika M

  Graduate School Of Bioagricultural Sciences Nagoya University

• Makoto Ojika

  Graduate School Of Bioagricultural Sciences Nagoya University

• Tsuge Takashi

  Graduate School Of Bioagricultural Sciences Nagoya University

• Ojika Makoto

  Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Qi Jianhua

  Coll. Of Pharmaceutical Sciences Zhejiang Univ.

• Qi Jianhua

  College Of Pharmaceutical Sciences Zhejiang University

• Kobayashi Tetsuo

  Graduate School Of Bioagricultural Sciences Nagoya University

• Oba Yuichi

  Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Suzuki Yoshihiro

  Graduate School of Bioagricultural Sciences, Nagoya University

• Qi Jianhua

  College of Pharmaceutical Sciences, Zhejiang University

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