Construction of a Bacterial Artificial Chromosome Library for a Myxobacterium of the Genus Cystobacter and Characterization of an Antibiotic Biosynthetic Gene Cluster
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概要
- 論文の詳細を見る
A bacterial artificial chromosome (BAC) library was constructed to isolate the biosynthetic gene cluster for the polyketide/peptide hybrid-type antibiotic cystothiazole A from the myxobacterium Cystobacter fuscus strain AJ-13278. Sequence analysis of a 63.9 kb contiguous region that encompasses the biosynthetic gene cluster (cta) led to the identification of a polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) hybrid gene cluster 32.1 kb in size, which consists of six open reading frames (ORFs), ctaB to ctaG, as well as downstream genes ctaJ and ctaK (1.0 and 0.9 kb, respectively) responsible for the final biosynthetic steps. The genes ctaB, ctaE, and ctaF encode PKSs, the genes ctaC and ctaG encode NRPSs, and ctaD encodes an NRPS-PKS hybrid enzyme. Disruption of ctaD impaired cystothiazole A production. Additionally, two downstream genes, ctaJ and ctaK, which encode a nitrilase and an O-methyltransferase, respectively, must be responsible for the final methyl ester formation in the cystothiazole A biosynthesis.
- 社団法人 日本農芸化学会の論文
- 2005-07-23
著者
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Suzuki Y
Exploratory Medicinal Sciences Pgrd Nagoya Laboratories Pfizer Pharmaceuticals Inc.
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Suzuki Yoshihiro
Graduate School Of Bioagricultural Sciences Nagoya University
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Sakagami Y
Nagoya Univ. Nagoya Jpn
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Sakagami Youji
Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Sakagami Youji
Graduate School Of Bio-agricultural Sciences Nagoya University
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Okada Masahiro
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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QI Jianhua
Graduate School of Bioagricultural Sciences, Nagoya University
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OJIKA Makoto
Graduate School of Bioagricultural Sciences, Nagoya University
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Qi Jianhua
Graduate School Of Bioagricultural Sciences Nagoya University
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Suzuki Yumiko
Exploratory Medicinal Sciences Pgrd Nagoya Laboratories Pfizer Pharmaceuticals Inc.
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FENG Zhiyang
Graduate School of Bioagricultural Sciences, Nagoya University
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TSUGE Takashi
Graduate School of Bioagricultural Sciences, Nagoya University
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OBA Yuichi
Graduate School of Bioagricultural Sciences, Nagoya University
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KOBAYASHI Tetsuo
Graduate School of Bioagricultural Sciences, Nagoya University
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Okada Masahiro
Dep. Of Chemistry Tohoku Univ.
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Oba Yuichi
Graduate School Of Bioagricultural Sciences Nagoya University
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Oba Yuichi
Graduate School Of Bioagricultural Sciences
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Feng Zhiyang
Graduate School Of Bioagricultural Sciences Nagoya University
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Ojika M
Graduate School Of Bioagricultural Sciences Nagoya University
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Makoto Ojika
Graduate School Of Bioagricultural Sciences Nagoya University
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Tsuge Takashi
Graduate School Of Bioagricultural Sciences Nagoya University
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Ojika Makoto
Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Qi Jianhua
Coll. Of Pharmaceutical Sciences Zhejiang Univ.
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Qi Jianhua
College Of Pharmaceutical Sciences Zhejiang University
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Kobayashi Tetsuo
Graduate School Of Bioagricultural Sciences Nagoya University
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Oba Yuichi
Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Suzuki Yoshihiro
Graduate School of Bioagricultural Sciences, Nagoya University
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Qi Jianhua
College of Pharmaceutical Sciences, Zhejiang University
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