Preparation and Characterization of Fully Active Biotinylated Analogs of Phytosulfokine-α
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概要
- 論文の詳細を見る
We report the preparation of biotinylated analogs of phytosulfokine-α (Tyr (SO_3H)-Ile-Tyr (SO_3H)-Thr-Gln ; PSK-α), an endogenous peptide growth factor in plants. Because the modification of the N-terminal amino group leads to significant loss of the activities, a Lys residue was incorporated in the C-terminal region of PSK-α, and its e amino group was reacted with biotinylation reagent. Results of the binding assay showed that [N^ε-(biotinyl) Lys^5] PSK-α retained the same binding activity and mitogenic activity as that of native PSK-α. Insertion of a single or double 6-aminohexanoic acid spacer between the ε amino group of Lys^5 and the carboxyl group of biotin did not significantly alter the activities of biotinylated [Lys^5] PSK-α. Structure-activity information obtained here would be useful for the detection and isolation of PSK-α receptors.
- 社団法人日本農芸化学会の論文
- 1999-10-23
著者
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Suzuki Yoshihiro
Graduate School Of Bioagricultural Sciences Nagoya University
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Matsubayashi Yoshikatsu
Laboratory of Bioactive Natural Products Chemistry, Graduate School of Bioagricultural Sciences, Nag
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Sakagami Youji
Laboratory of Bioactive Natural Products Chemistry, Graduate School of Bioagricultural Sciences, Nag
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Sakagami Y
Nagoya Univ. Nagoya Jpn
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Sakagami Y
Nagoya Univ.
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Matsubayashi Yoshikatsu
School Of Agricultural Sciences Nagoya University
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Morita A
Laboratory Of Bioactive Natural Product Chemistry Graduate School Of Bio-agricultural Sciences Nagoy
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Takahata Yoshinori
Laboratory Of Bioactive Natural Products Chemistry Graduate School Of Bio-agricultural Sciences Nago
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Morita Akiko
Laboratory of Bioactive Natural Product Chemistry, Graduate School of Bio-Agricultural Sciences, Nag
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ATSUMI Keisuke
Laboratory of Bioactive Natural Products Chemistry, Graduate School of Bio-Agricultural Sciences, Na
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Morita Akiko
Laboratory Of Bioactive Natural Product Chemistry Graduate School Of Bio-agricultural Sciences Nagoy
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Atsumi Keisuke
Laboratory Of Bioactive Natural Products Chemistry Graduate School Of Bio-agricultural Sciences Nago
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Sakagami Youji
Laboratory Of Bioactive Natural Product Chemistry Graduate School Of Bio-agricultural Sciences Nagoy
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Suzuki Yoshihiro
Graduate School of Bioagricultural Sciences, Nagoya University
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