Stereoselective Incorporation of Isoleucine into Cypridina Luciferin in Cypridina hilgendorfii (Vargula hilgendorfii)
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概要
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The emission of light in the marine ostracod Cypridina hilgendorfii (presently Vargula hilgendorfii) is produced by the Cypridina luciferin-luciferase reaction in the presence of molecular oxygen. Cypridina luciferin has an asymmetric carbon derived from isoleucine, and the absolute configuration is identical to the C-3 position in L-isoleucine or D-alloisoleucine. To determine the stereoselective incorporation of the isoleucine isomers (L-isoleucine, D-isoleucine, L-alloisoleucine, and D-alloisoleucine), we synthesized four 2H-labeled isoleucine isomers and examined their incorporation into Cypridina luciferin by feeding experiments. Judging by these results, L-isoleucine is predominantly incorporated into Cypridina luciferin. This suggests that the isoleucine unit of Cypridina luciferin is derived from L-isoleucine, but not from D-alloisoleucine.
- 2006-06-23
著者
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OBA Yuichi
Graduate School of Bioagricultural Sciences, Nagoya University
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Oba Yuichi
Graduate School Of Bioagricultural Sciences Nagoya University
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Oba Yuichi
Graduate School Of Bioagricultural Sciences
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INOUYE Satoshi
Yokohama Research Center, Chisso Co.
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Inouye Satoshi
Yokohama Research Center Chisso Co.
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Inouye Satoshi
Yokohama Res. Center Chisso Corp.
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Ojika Makoto
Graduate School Of Bioagricultural Sciences Nagoya Univ.
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KATO Shin-ichi
Graduate School of Bioagricultural Sciences, Nagoya University
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