Comparison of Luminescent Immunoassays Using Biotinylated Proteins of Aequorin, Alkaline Phosphatase and Horseradish Peroxidase as Reporters

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概要

• 論文の詳細を見る

• We compare aequorin, alkaline phosphatase and horseradish peroxidase as reporters for luminescent immunoassays using α-fetoprotein (AFP) as a model analyte. Biotinylated aequorin was prepared from mutated aequorin containing a reactive cysteine residue by chemical conjugation. The measurable range of AFP using this biotinylated aequorin was 0.02–200 ng/ml, with a lower background level than the other biotinylated enzymes tested.

• 社団法人 日本農芸化学会の論文
• 2008-12-23

著者

• Inouye Satoshi

  Yokohama Res. Center Chisso Corp.

• SATO Jun-ichi

  Yokohama Research Center, Chisso Corporation

• Sato Jun-ichi

  Yokohama Res. Center Chisso Corp.

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