Blarina toxin, a mammalian lethal venom from the short-tailed shrew Blarina brevicauda : Isolation and characterization
スポンサーリンク
概要
- 論文の詳細を見る
Venomous mammals are rare, and their venoms have not been characterized. We have purified and characterized the blarina toxin (BLTX), a lethal mammalian venom with a tissue kallikrein-like activity from the submaxillary and sublingual glands of the short-tailed shrew Blarina brevicauda. Mice administered BLTX i.p. developed irregular respiration, paralysis, and convulsions before dying. Based on the amino acid sequence of purified protein, we cloned the BLTX cDNA. It consists of a prosequence and an active form of 253 aa with a typical catalytic triad of serine proteases, with a high identity with tissue kallikreins. BLTX is an N-linked microheterogeneous glycoprotein with a unique insertion of 10 residues, L106TFFYKTFLG115. BLTX converted kininogens to kinins, which may be one of the toxic pathogens, and had dilatory effects on the blood vessel walls. The acute toxicity and proteolytic activity of BLTX were strongly inhibited by aprotinin, a kallikrein inhibitor, suggesting that its toxicity is due to a kallikrein-like activity of the venom.
- The National Academy of Sciencesの論文
- 2004-05-18
著者
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大舘 智氏
北大低温研
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北 将樹
名大院理
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大舘 智志
北海道大学低温科学研究所
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Ohdachi S
Institute Of Low Temperature Science Hokkaido University
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Ohdachi Satoshi
Hokkaido Univ. Sapporo Jpn
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Ohdachi Satoshi
Institute Of Low Temperature Science Hokkaido University
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Kita Masaki
Research Center for Materials Science
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Nakamura Yasuo
Institute for Enzyme Research, The University of Tokushima
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Yoshikuni Michiyasu
National Institute for Basic Biology
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Kido Hiroshi
Institute for Enzyme Research, The University of Tokushima
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Uemura Daisuke
Department Of Chemistry Graduate School Of Science
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Uemura Daisuke
Graduate School Of Science And Institute For Advanced Research Nagoya University
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Uemura Daisuke
Faculty Of Liberal Arts Shizuoka University
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Ohdachi Satoshi
Institute Of Low Temperature Science
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Ohdachi Satoshi
Zoological Section Institute Of Low Temperature Science Hokkaido University
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Ohdachi Satoshi
Laboratory Of Applied Zoology Faculty Of Agriculture Hokkaido University
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Yoshikuni M
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Oba Yuichi
Graduate School Of Bioagricultural Sciences
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Kido Hiroshi
Institute For Enzyme Research The University Of Tokushima
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Kido H
Univ. Tokushima Tokushima Jpn
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Nakamura Yasuo
Institute For Enzyme Research The University Of Tokushima
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Okumura Yuushi
Institute For Enzyme Research The University Of Tokushima
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上村 大輔
名古屋大学大学院理学研究科
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Uemura Daisuke
Fancl Res. Inst. Fancl Corp.
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Yoshikuni M.
Reprod. Biol. Natl. Inst. For Basic Biol. Okazaki
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