Reporter Gene Analysis of AKT3-1 and AKT3-2 Expression During Conidial Germination of the Japanese Pear Pathotype of Alternaria alternata
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概要
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The Japanese pear pathotype of Alternaria alternata produces the host-specific AK-toxin and causes black spot of Japanese pear. AK-toxin is produced by mycelia grown in nutritional media and by conidia germinated in water. We have previously identified two homologous genes, AKT3-1 and AKT3-2, in the genomic region controlling toxin biosynthesis. From gene-targeting experiments, we demonstrated that AKT3-2, at least, is essential for toxin biosynthesis. However, functional characterization of AKT3-1 was unsuccessful, because of the presence of multiple copies in the genome. Expression of these genes was studied in strains transformed with vectors p3-1GUS-2 and p3-2GUS-2, which contain the β-glucuronidase (GUS) reporter gene fused to the AKT3-1 and AKT3-2 promoters, respectively. GUS activity was detected in mycelia of p3-1GUS-2 and p3-2GUS-2 transformants grown in potato dextrose broth, indicating that AKT3-1 and AKT3-2 both are expressed under optimal growth conditions in an artifical medium. Because the toxin is not stored in dormant conidia, early expression of the toxin biosynthetic genes during conidial germination is crucial for successful invasion and colonization of susceptible leaf tissues. In cytological observation of germinated conidia on cellulose membranes, stable GUS activity was detected in germ tubes of both transformants during germtube emergence and elongation, showing the transcription of AKT3-1 and AKT3-2 during an early stage of infection.
- 日本植物病理学会の論文
- 2001-02-25
著者
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TSUGE Takashi
Graduate School of Bioagricultural Sciences, Nagoya University
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Tanaka Aiko
Graduate School Of Bioagricultural Sciences Nagoya University
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Tsuge Takashi
Graduate School Of Bioagricultural Sciences Nagoya University
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