ハシリドコロ培養組織の生長ならびにアルカロイド生成

概要

論文の詳細を見る
Callus tissue was obtained from the rhizome explant of Scopolia japonica MAXIM. cultured on the Linsmaier & Skoog's synthetic medium supplemented with 0.22 ppm 2,4-D. For the subculturing of callus, the addition of 2,4-D to the medium was essential and it could not be substituted by IAA. Callus cultures showed a very low IAA oxidase activity, and the growth was strongly inhibited by a series of phenylpropane compounds (50〜2000 μM) derived from shikimic acid. The amount of tropane alkaloids produced by the callus varied greatly from 0.004 to 0.089 % on the dry weight basis depending upon culture conditions. Among the cultures grown on the synthetic medium in the dark at 25℃, the one supplemented with 0.22 ppm of 2,4-D and 2.0 ppm of kinetin showed the highest alkaloid content. Also, the addition of either casein hydrolysate or peptone to the basal medium was effective to increase the alkaloid content. The thin layer chromatography of the alkaloids extracted from the callus suggested the presence of hyoscine, whereas hyoscyamine was not detected by this analysis. Besides, three alkaloidal spots which were not detected in the extract of the rhizome were found on the chromatogram.
日本生薬学会の論文
1967-12-15