Prunus属種子類生薬の研究(第3報) : 免疫電気泳動法による種子たんぱくの比較研究

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Seed proteins of 13 Prunus species (Prunoideae) and 4 species of Pomoideae were studied by the immunological method using a rabbit antiserum to the Japanese apricot (P. armeniaca L. var, ansu MAXIM.). In general, very similar protein compositions were found among the tested Prunus species. Thus, many of the species could not be distinguished from each other by the number of precipitin lines but by the pattern of lines. As for the species belonging to Prunoideae, 7 lines were observed in P. armeniaca L. var. ansu MAXIM. and P. mandschurica KOEHNE, whereas 5-6 identical lines were shown by 10 species including P. persica BATCH, P. mume SIEB., P. mume SIEB. var. bungo MAKINO, P. salicina LINDL., P. amygdalus STOKES var. dulcis DE CANDDOLE, P. japonica THUNB., P. tomentosa THUNB., P. yedoensis MATSUM., P. jamasakura SIEB., P. grayana MAXIM. However, only two lines were formed by P. caroliniana AIT., which once belonged to the genus Padus. On the other hand 4 species of Pomoideae (Eriobotrya japonica LINDL., Malus pumila MILL, Malus micromalus MAKINO, Cydonia sinensis THOUIN.) showed only one weak line. The two precipitin lines in P. caroliniana AIT. and the one in Pomoideae were found to be immunochemically identical with certain proteins in the Japanese apricot. As a remarkable difference in the pattern was found between apricot and peach seeds, these two important crude drugs can be distinguished easily by immunoelectrophoresis. Although the immunoelectrophoresis is useful for studying the systematic relationship between Prunoideae and Pomoideae, the disc electrophoresis previously reported seems to be more adequate for a more detailed study on specific differences.
日本生薬学会の論文
1971-06-20

Prunus属種子類生薬の研究(第3報) : 免疫電気泳動法による種子たんぱくの比較研究

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