P-7 放線菌の生産するテルペノイドに関する研究(ポスター発表の部)

概要

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Most Streptomyces strains are equipped with only the MEP (2-C-methyl-D-erythritol 4-phosphate) pathway for the formation of IPP (isopentenyl diphosphate). In addition to this pathway. some Streptomyces strains use the tnevalonate pathway to produce secondary metabolites. Streptomyces sp. KO-3988 was the first example to have "two" distinct mevalonate gene clusters. Our investigations had shown that the organism possessed "two" mevalonate pathway gene clusters with one cluster being responsible for production of antitumor agents. the furaquinocins. Subsequent gene analysis of the second mevalonate pathway gene cluster showed that it was flanked by the GGDP (geranylgeranyl diphosphate) synthase gene at its upstream region. Since GGDP is the precursor of diterpenes, the strain was assumed to produce diterpene compounds that had never been isolated from this strain. We thus attempted to isolate diterpenes from fermentation broth of this strain and succeeded in purifying five novel diterpenes named oxaloterpins A (1), B (2), C (3), D (4) and E (5) together with a previously-described compound. viguiepinone (6). Their structures were established based on extensive NMR and mass spectral analyses. The absolute configurations of oxaloterpins were determined by the modified Mosher's method.
天然有機化合物討論会の論文
2007-08-24