35 抗生物質Dityromycinの構造(口頭発表の部)

概要

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Antibiotic, dityromycin was isolated from the culture broth of Streptomyces sp. strain No. AM-2504. It is active against Bacillus, Corynebacterium, and Clostridium, but inactive against Staphylococcus aureus and Gram-negative bacteria. Dityromycin was determined to have a molecular weight of 1288 by FAB-MS, and to be composed of N-MeVal (2), Pro (2), Val (1), phenylglycine (1), and CH_3NH_2 (1) (6M HCl, 140℃, 40h). In addition, N,N-Me_2Thr-Val-OH (1) was isolated from the hydrolyzate with 6M HCl (110℃, 24h) and di-α-amino acid (3) containing diphenylether and γ-hydroxyisoleucine lactone (4) were obtained by reductive hydrolysis with 57% HI-P (110℃, 24h). The sequence of peptide fragments, 5 and 6, obtained by alkaline treatment (1M NaOH, r.t., 3h) were clarified by partial hydrolysis, selective cleavage, and Edman degradation. Moreover, another fragment peptide 7 whose structure was determined as shown in Fig. 4 was derived by partial acid hydrolysis (cHCl-AcOH (1: 1), 37℃, 3h). From the comparison of 7 with dityromycin (Table 5), the total structure could be deduced as shown in Fig. 6. Dityromycin was clarified to have unique structure which includes bicyclic depsipeptide, epoxide, diphenylether and so on.
天然有機化合物討論会の論文
1987-07-25