P-41 Trichoderma属糸状菌の生産する新規抗インフルエンザウイルス物質wickerols(ポスター発表の部)

概要

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Influenza virus infection is widespread and often critical for patients with respiratory diseases, immunosuppressive syndromes, aged persons with cardiopulmonary diseases and so on. Recent human infections caused by highly pathogenic avian influenza virus posed a pandemic threat. During screening for anti-influenza virus compounds from microbial origins, a novel antibiotic, wickerols, produced by a fungus, Trichoderma atroviride FKI-3849, isolated from a soil sample from Hawaii Island, Hawaii, USA, exhibited anti-viral properties. The strain was cultured in Erlenmeyer flasks on a rotary shaker at 28℃ for 4 days. Six liters of the cultured broth was extracted by ethyl and then extracted by ethyl acetate. This was chromatographed over a silica gel column, and 18.1mg of wickerol A and 3.2mg of wickerol B were obtained as white powders. The structures of wickerol A and B were elucidated by NMR study. They have a unique 3H-5a,9-methanocycloocta[cd]indene skeleton (fused 6-5-6-6 ring system). The structures were confirmed by X-ray crystallographic analysis. Wickerol A inhibited replication of mouse-adapted influenza virus A/PR/8/34 in Madin-Darby canine kidney (MDCK) cells at an IC_<50> value of 0.07μg/ml, while it inhibited the growth of MDCK cells at an IC_<50> value of 7μg/ml. Wickerol B showed week anti-influenza acitivity compared with wickerol A.
2008-09-01