81(P-81) 放線菌におけるメバロン酸経路の有無とイソプレノイド化合物生産性との関連について(ポスター発表の部)

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Isoprenoid compounds are derived from a five-carbon precursor, isopentenyl diphosphate (IPP), which is known to be synthesized via mevalonate and/or 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway(s). Although most of bacteria including Streptomyces strains are equipped with only the MEP pathway for the formation of IPP, we previously and clearly showed that both the mevalonate and MEP pathways were simultaneously operating in some Streptomyces strains. Kitasatospora griseola, one of such strains, is known to produce a diterpene antibiotic terpentecin (TP). We have previously and clearly shown that a TP biosynthetic gene cluster was located in adjacent of the mevalonate pathway gene cluster. In this study, therefore, we examined whether the mevalonate pathway genes and isoprenoid compound biosynthetic genes are usually clustered in a genomic DNA region in the strains possessing both the mevalonate and the MEP pathways. A mevalonate pathway gene cluster was successfully cloned from Actinoplanes sp. strain A40644, which was known to possess both of pathways and produce an isoprenoid compound, BE-40644, by hybridization using an HMG-CoA reductase gene cloned from the TP producer as a probe. By sequencing flanking regions, a probable BE-40644 biosynthetic gene cluster was suggested to exist in a downstream region of the mevalonate pathway gene cluster. Heterologous expression of a 9-kb fragment confirmed that a set of the BE-40644 biosynthetic genes was involved in the fragment. These results suggested that the presence or the absence of the mevalonate pathway might be a good landmark to detect the production of isoprenoid compounds by actinomycetes. Tetrahedron Lett., 37, 7979 (1996), Tetrahedron Lett., 39, 9497 (1998), Biosci. Biotech. Biochem., 66, 808 (2002), J. Bacteriol., 183, 6085 (2001), J. Antibiot., 45, 957 (2003).
天然有機化合物討論会の論文
2004-10-01

81(P-81) 放線菌におけるメバロン酸経路の有無とイソプレノイド化合物生産性との関連について(ポスター発表の部)

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