44 複雑な化合物の構造研究に有用なNMRの測定方法-3D-HMBC(口頭発表の部)

概要

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In order to overcome the problems of HMBC associated with the setting of delay time for observing small long range couplings and short T_2 with broad methylene proton signals, we have developed a new technique, 3D-HMBC. In the pulse sequence of this method, delay time (Δ), which has to be fixed to an appropriate value (typically 60msec) in the conventional 2D-HMBC, is replaced by a variable parameter (t_1). This modification enables to cover wide delay time range needed to observe from small to large long range couplings and thus gives better HMBC spectra than obtained by the conventional method. In addition, we have developed a new NMR pulse sequence (3D-J-HMBC) useful for observing indirectly long range ^<13>C-^<13>C couplings separated by two bonds by modification of the 3D-HMBC. Its application for biosynthetic studies of a fungal metabolite, agonomic acid is explained.
天然有機化合物討論会の論文
1997-07-20