Colorimetric Assay of Diamine Oxidase Activity with Histamine as the Substrate(Analytical,Chemical)
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概要
- 論文の詳細を見る
A simple method for the determination of diamine oxidase(DAO) activity is described. The method is based on oxidation of histamtne with DAO, formation of histamine-copper(II)chelated cation from the remaining histamine, and extraction of the ion associated with tetrubromophenolphthalein ethyl ester. A linear calibration curve between absorbance and DAO activity was obtained for DAO activity up to 250mU/ml. The inter-assay coefficient of variation was 2.12% with a recovery of 101.7% at the DAO activity of 123.5 mU/ml. A close correlation was found between the results of this method and the 2,2'-azmo-di(3-ethylben7Jhiazoline-6-sulfonic acid) method.
- 公益社団法人日本薬学会の論文
- 1987-07-25
著者
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高柳 美行
愛知県赤十字血液センター
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八代 有
Faculty of Pharmaceutical Sciences Nagoya City University
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後藤 鉦二
愛知県赤十字血液センター
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八代 有
名古屋市立大学薬学部
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高柳 美行
Aichi Prefecture Red Cross Blood Center
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後藤 鉦二
Aichi Prefecture Red Cross Blood Center
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山上 由加里
Faculty of Pharmaceutical Sciences, Nagoya City University
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内藤 敏子
Faculty of Pharmaceutical Sciences, Nagoya City University
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内藤 敏子
Faculty Of Pharmaceutical Sciences Nagoya City University
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山上 由加里
Faculty Of Pharmaceutical Sciences Nagoya City University
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