Determination of Acetaldehyde in Human Blood by High-Performance Liquid Chromatography Using Fluorometry

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概要

• 論文の詳細を見る

• A method was developed for the measurement of acetaldehyde in human blood by high-performance liquid chromatography (HPLC). The method was based on a pre-column reaction; a fluorescent substance was formed by a coupling reaction between 2mol of cyclohexan-1,3-dione and 1 mol of acetaldehyde with ammonium acetate. The coupling compound was analyzed by HPLC and the concentration of acetaldehyde was obtained from the calibration curve drawn from the results using standard solutions. The determination was sensitive and reproducible with a range of 0.2-10 μM and a precision (coefficient of variation) of 2.43%.

• 公益社団法人日本薬学会の論文
• 1989-01-25

著者

• 高柳 美行

  愛知県赤十字血液センター

• 小久保 幸雄

  愛知県赤十字血液センター

• 八代 有

  Faculty of Pharmaceutical Sciences Nagoya City University

• 後藤 鉦二

  愛知県赤十字血液センター

• 八代 有

  名古屋市立大学薬学部

• 鈴木 誠

  Department Of Pharmacy Nagoya Post And Telecommunication Hospital

• 高柳 美行

  Aichi Prefecture Red Cross Blood Center

• 後藤 鉦二

  Aichi Prefecture Red Cross Blood Center

• 小久保 幸雄

  Aichi Prefecture Red Cross Blood Center

• 鈴木 誠

  Faculty of Pharmaceutical Sciences, Nagoya City University

• 八代 有

  名古屋市立大学

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